A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. Next generation SpectraMax® absorbance microplate readers. Transfected cells in the 6-well plate were trypsinized, divided into ten aliquots per well, pelleted at 1500 rpm for 5 minutes, and washed once with PBS. A kinetic plate read was initiated at time zero. Built-in near-field communication (NFC) reader enables you to pull up your custom protocols and results with a single tap, saving you precious time. Feb 22, 2023Fast and compact absorbance microplate readers for a wide range of assays without the use of filtersSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. Assay flexibility. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Microscopes and Microplate Instrumentation. Fluorescence Microplate Reader Comparison Chart. Step 1: Open the SoftMax Pro IMAP FP protocol. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 1 sec. If it looks fine, then proceed. 306 plate air cal. ABS2 Validation Plate Protocol Installation InstructionsEndpoint, dual read, kinetic, fast kinetic. Read one sample or up to. 1. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. 9. The sample is typically placed in a cuvette through which light is sent horizontally. 9. To update the driver. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. 190. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Learn more about their features and specifications in. An industry-renowned instrument for monitoring GPCRs and ion channels. To update the driver. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Figure 1. GxP. Products : General, FilterMax F5 reader. #P7589 or P11496) • Black 96-well plate (Greiner Bio-One, cat. , attenuation mode = out. 1700 Online: Sales Offices USA/Canada 800. Microplate Stacker and Washer. Category 2 and 3 microplate readers use gradient heating as well. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. , Sunnyvale, CA 94089, USA, Jin_Liao@moldev. HTRF-compatible SpectraMax Multi-Mode Readers. These systems are hybrids that allow you to choose between. Vmax® plate reader specifications. Plate Formats: 6 to 384-well microplates. +1 800-635-5577. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. This process should take about 3 - 5 minutes. San Jose, California, June 5, 2018. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. No accessories are needed and SoftMax® Pro Software. Details. View the Molecular Devices website. 0077 OD 0. If the computer is connected to the Internet: Select Search Automatically for. To update the driver. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. FlexIII. S. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. SpectraMax MiniMax 300 imaging cytometer enables quick imaging and analysis of cells and helps in observing the viability of your cells with the field-upgradeable cellular imaging for spectramax i3/i3x multi-mode detection platform. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SoftMax Pro’s 21 curve fit options, including 4-parameter logistic and 5-parameter logistic models, and the ability to assign multiple standard curves within the same plate make it easy to interpolate unknown samples or perform parallel line analysis (PLA) to determine relative potency. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. a single plate read using any standard cuvette, or 96- or 384-well microplate. SAN JOSE, Calif. Recertification service. 635. Click on each for more information. Step 4: Incubate at room temperature for 1 hour, protected from light. 0 . Ensure data accuracy of your reader. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Molecular Devices' microplate readers. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. For biochemical assays, the washer removes unbound material and unreacted reagents. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. SpectraMax® Microplate Reader Suite of Products Knowledge Base. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. visible absorbance, fluorescence. 944. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 8 foot) 4400-0002 Power cord, 1 meter (3. Microplate Readers. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. With three modes of detection for full spectrum absorbance, fluorescence and luminescence, the SpectraMax® Mini reader . The automated ELISA workflow has two positive outcomes. Feb 22, 2023FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Eight filter options provide simplified assay setup. Superior opticsThe top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. Tests every subsystem of the reader or . Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic. Support. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Read times. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 2. Monday to Friday 7am - 5pm Pacific Time. SoftMax Pro GxP Software. Page 17 Chapter 2: Setting Up the Instrument 4. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. Molecular Devices' microplate readers. 2. The plate reader optics are tunable for absorbance,. ~ Bio-Tek ELx808 Microplate Reader $2,795. 0 OD for earlier models. Fill a clean plate with water. Chapter 1: Introduction The SpectraMax iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. 3 foot) See. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software Check software compatibility with Windows 10 and locate your software installer. The Gemini instruments can read some luminescence but it is about 100 times less sensitive than a dedicated luminometer. These lab workhorses are used in drug discovery. The new assay will complement Molecular Devices’ workflow solution for cell line development by offering a 10-minute titer assay that can be run on the company’s SpectraMax series of microplate. Turn on Plate Reader and wait for it to complete its start-up routine. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The primary mode is fluorescence. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SoftMax Pro 7. V. Plus 384. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. SpectraTest Multi-Mode Validation Plate User Guide SpectraTest Validation Plates and SoftMax Pro Software reader validation protocols. M5. Select the Driver tab. Uncontrolled Copy for Training and Reference Purposes Only . We would like to show you a description here but the site won’t allow us. User selects appropriate wells for samples and control using plate configuration tool. 7°C (gradient heating design). An ampicillin-resistant E. Eight filter options provide simplified assay setup. 1. The procedures for these tests are outlined in the instrument user manual. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 2. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. 340 PC384. Cellular Imaging Systems: Molecular Devices offers various imaging solutions for your research needs. SpectraMax® Microplate Readers and SoftMax® Pro Software. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Increase productivity with patented microplate reader technologies. 0 . With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well. and fluorescence microplate readers—. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 0200-6262. Chapter 1: Introduction The SpectraMax® iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. FlexStation™ II Systems provide integrated fluid transfer capability from a source platePlate Reader SpectraMax iD5 multi-mode system Plate Washer AquaMax 4000 Microplate Washer Robot PreciseFlex 400 Sample Handler Plate Storage Plate Hotels – 40 microtiter plates Controlling Software Genera Control Software Incubation LiCONiC STX44 Automated CO 2 Incubator Liquid Handler Microlab STAR Automated Liquid Handling The. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. 1. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. 312 gain calibration failed. One plate carrier adapter is provided with the instrument. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 3T3 L1 adipocytes were plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours,Lower variance: Normalize your plate reader assay data to the number of cells in each well. ] Example of document to evaluate if the reader . Figure 9. S. D. 9. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Fibroblasts vs. . Complete plate reader solution for all your research needs. 1-1. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. SpectraMax Microplate Readers. 314 low lamp level warning. 0A Sunnyvale, CA Made in USAFor SpectraMax ABS Plus reader, r 2 = 0. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. This document is provided to customers who have purchased Molecular Devices, Inc. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. Reader Documentation Version No. Contact our support team with your questions regarding Molecular Devices products and applications. ; SoftMax® Pro 7. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. book Page 1 Tuesday, July 6, 2010 2:08 PM SpectraMax Microplate Readers. was added and absorbance was measured at 450 nm and 650 nm using a plate reader (Molecular Devices, SpectraMax ABS Plus, SoftMax Pro 7. ®This document is provided to customers who have purchased Molecular Devices, Inc. 00. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Important things to consider when purchasing a multimode microplate reader. Cell viability는 세포막 Integrity 또는. 2. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Our 15 years of experience means we understand these instruments as well as the manufacturer, and it allows us to provide top-quality service. 1). ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 1. 1. 0200-6263. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Integrated to. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0. (a) Chemical structures of 1 (HADP) and its analogues 2–4. If the computer is connected to the Internet: Select Search Automatically for. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. SpectraMax Readers have the most complete level of product validation and compliance. Tristar 3 Multimode Microplate Reader. If no sound is heard during initialization, contact technical support. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great. , target CV per well = 1. Cellular Imaging Systems. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Open the top cover of the instrument. 10,700. Plate pre-read measurements were made in a Costar UV plate. Published Dec 16, 2020 | Updated Nov 08, 2023. Luminescence is the emission of light by a substance as a result of a chemical reaction (chemiluminescence) or an enzymatic reaction (bioluminescence). SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. SpectraMax® Microplate Readers and SoftMax® Pro Software. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Tutorial on how to quickly and easily view your results on the SpectraMax iD3 Multi-Mode Microplate Reader. For nearly 40 years, we have partnered with scientists to help them achieve landmark discoveries. #655096) • Brown or amber (light-blocking microcentrifuge tubes • Molecular Devices microplate reader with fluorescence detection mode:What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Molecular Devices offers a broad range of technologies for high-throughput screening, genomic and cellular analysis, colony selection, and microplate detection. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Expanded wavelength coverageAre you looking for a versatile and affordable microplate reader that can perform various assays and measurements? Check out the FilterMax F3 and F5 Multi-Mode Microplate Readers, which offer absorbance, fluorescence, luminescence, and more read modes with high performance and reliability. 3. #S1207. Versa Max. The plate reader optics are. ensure your reader is thoroughly tested against specifications we consider critical to achieving quality results. 2. The Synergy H4 is a convenient benchtop microplate reader that offers robust. Molecular Devices SpectraMax® microplate readers support a multitude of assays for nucleic acids and protein detection. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Absorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. Figure 10: The Plate section Template Editor, showing the series set up. 384 deep well plate: packed in sleeves of 6 , 24 per case. For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. Fluorescence Microplate Reader Comparison Chart. Select Update Driver. 40266 Email: drugdiscoverytech@lifetech. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular. However, when a specific probe is not available, or it causes off-target effects on the. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Single-mode Microplate Reader. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. When the user applies a set temperature of 37°C, the top is heated to 37. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Cellular Imaging Systems. The plate reader. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 0 . An example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. Turn the reader on and secure the USB cable. 1. Clone Screening. Published Dec 16, 2020 | Updated Dec 06, 2021. The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in. Important things to consider when purchasing a multimode microplate reader. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. 1-2 sec. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535. Microplate readers and controlling software enable walk away data analysis. Access all the resources, tools and information you need to stay productive. . The plate was placed on a shaker for 30 seconds and then incubated for 3 hours at 37°C, 5% CO 2. It was then read from the bottom on a SpectraMax ® i3 Multi-Mode Microplate Reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. 304 signal level saturation. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Shop now. EUROPE. 1. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). It then uses the pre-defined settings for the specified instrument to build a protocol. We would like to show you a description here but the site won’t allow us. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. Combined with the analysis capabilities of SoftMax® Pro Software, we provide quantitation in an easy-to-read, user-customizableThe SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. In an analogous manner, the same sample names were assigned to a CuvetteSet section. generation SpectraTest ABS2 Absorbance Validation Plate. Exploring misconceptions and understanding the technologies. coli stock with an OD 600 value of 0. MycoAlert Substrate was then added to the wells, and the plate was incubated for 10 minutes at room temperature. M2. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. Plate section without PathCheck applied. Evaluation of BioTek's Synergy H4 Plate Reader for Fluorescent Detection. The lower limit of detection (LLD) was defined as the level of. It may be named "Pothos". The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. ABS Plus can run both cuvette-based and microplate reader applications on the same instrument. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Instrument settings can be saved as a protocol file. This page contains the. Purchase a new SpectraMax Multi-Mode Microplate Reader and. Flexibility to read a variety of plate formats. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemPrimary considerations when choosing a microplate reader are throughput and flexibility. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. #Flex3) Methods. The former have been the traditional domain of plate readers and great strides have been. 6% for the on-board optics (monochromators). 5 mm hex key to loosen, but not remove, the hex screw that secures the filter to the filter slide. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. We would like to show you a description here but the site won’t allow us. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. #X7060. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Eliminate time-dependent substrate addition steps. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The adapter is required for optimum performa nce with standard 96-well and 384-. W×D×H. ®ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. Recombinant protein expression Nucleocapsid. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. It features a modular design that allows users to add or change detection cartridges according to their needs. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. SpectraMax ABS and ABS Plus Microplate Readers are a compact and tunable absorbance microplate readers. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Step 3. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. Multi-mode Plate Reader. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. STAKMAX. Fluorescence Microplate Reader Comparison Chart. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. After reading the ELISA plate on both readers, each standard curve was nearly identical. In this unique new five-part weekly webinar series, running every Thursday throughout October 2020, we’ll introduce you to various aspects of microplate reader technology in an educational and entertaining way! Starting with some basic concepts,. 5. Timothy Bolus, MPM, MBA, Compliance Program Manager. Increased throughput. White plates reflect the light, enabling even low levels of Luminescence to be detected. Multimode Microplate Readers. 400×320×350 mm. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. The SpectraMax ABS. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Select the Driver tab. The temperature in the plate chamber is ambient and isothermal. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. View SoftMax Pro GxP. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U.